4/17/2023 0 Comments Prepros importYeast carboxypeptidase Y can be translocated and glycosylated without its amino-terminal signal sequence. Genetic analysis of protein export in Escherichia coli K12. Expression of genes in yeast using the ADCI promoter. Nucleotide sequence of the triose phosphate isomerase gene of Saccharomyces cerevisiae. Links to PubMed are also available for Selected References. Get a printable copy (PDF file) of the complete article (1.8M), or click on a page image below to browse page by page. Full textįull text is available as a scanned copy of the original print version. Two of three mutants tested produced lower steady-state levels of alpha-factor in intact yeast cells, although the magnitude of the effect was less than that in the cell-free system. After reassembly into an intact alpha-factor gene, the substitutions were found to decrease up to 50-fold the rate of translocation of prepro-alpha-factor across microsomal membranes in vitro. The mutations resulted in replacement of a residue in the hydrophobic core of the signal sequence with either a hydrophilic amino acid or a proline. Signal sequence mutants were obtained by selecting for cells that partially mistargeted into mitochondria a fusion protein consisting of the alpha-factor signal sequence fused to the mature portion of an imported mitochondrial protein (Cox IV). We used a genetic approach to identify point mutations in the signal sequence of a secreted eucaryotic protein, yeast alpha-factor.
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